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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agroenergia. Para informações adicionais entre em contato com cnpae.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
29/09/2015 |
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Data da última atualização: |
20/09/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
SANTOS, D. F. K. dos; ISTVAN, P.; NORONHA, E. F.; QUIRINO, B. F.; KRÜGER, R. H. |
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Afiliação: |
Débora Farage Knupp dos Santos, UnB; Paula Istvan, UnB; Eliane Ferreira Noronha, UnB; BETANIA FERRAZ QUIRINO, CNPAE; Ricardo Henrique Krüger, UnB. |
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Título: |
New dioxygenase from metagenomic library from Brazilian soil: insights into antibiotic resistance and bioremediation. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Biotechnology Letters, v. 37, n. 9, p.1809-1817, 2015. |
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DOI: |
10.1007/s10529-015-1861-x |
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Idioma: |
Inglês |
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Conteúdo: |
Objectives Putative new dioxygenases were identified in a metagenomic b-lactam-resistance screening and, given their key role on aromatic metabolism, we raise the hypothesis that these enzymes maybe concomitantly related to antibiotic resistance and aromatic degradation. Results ORFs of three putative dioxygenases were isolated from resistant metagenomic clones. One of them, CRB2(1), was subcloned into pET24a expression vector and subjected to downstream phenotypic and bioinformatics analyses that demonstrated the ??dual effect?? of our metagenomic dioxygenase, on antibiotic and aromatic resistance. Furthermore, initial characterization assays strongly suggests that CRB2(1) open-reading frame is an extradiol-dioxygenase, most probably a bicupin domain gentisate 1,2-dioxygenase. This observation is, to our knowledge, the first description of a metagenomic dioxygenase and its action on b-lactam resistance. Conclusion Unraveling the diversity of antibiotic resistance elements on the environment could not only identify new genes and mechanisms in which bacteria can resist to antibiotics, but also contribute to biotechnology processes, such as in bioremediation. |
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Palavras-Chave: |
Aromatic metabolism; Aromatic metabolismo; Dioxygenase; Dioxygenase Metagenome; Metagenome; Resistência aos antibióticos. |
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Thesagro: |
Solo. |
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Thesaurus NAL: |
antibiotic resistance; soil. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02061naa a2200289 a 4500
001 2025343
005 2017-09-20
008 2015 bl uuuu u00u1 u #d
024 7 $a10.1007/s10529-015-1861-x$2DOI
100 1 $aSANTOS, D. F. K. dos
245 $aNew dioxygenase from metagenomic library from Brazilian soil$binsights into antibiotic resistance and bioremediation.$h[electronic resource]
260 $c2015
520 $aObjectives Putative new dioxygenases were identified in a metagenomic b-lactam-resistance screening and, given their key role on aromatic metabolism, we raise the hypothesis that these enzymes maybe concomitantly related to antibiotic resistance and aromatic degradation. Results ORFs of three putative dioxygenases were isolated from resistant metagenomic clones. One of them, CRB2(1), was subcloned into pET24a expression vector and subjected to downstream phenotypic and bioinformatics analyses that demonstrated the ??dual effect?? of our metagenomic dioxygenase, on antibiotic and aromatic resistance. Furthermore, initial characterization assays strongly suggests that CRB2(1) open-reading frame is an extradiol-dioxygenase, most probably a bicupin domain gentisate 1,2-dioxygenase. This observation is, to our knowledge, the first description of a metagenomic dioxygenase and its action on b-lactam resistance. Conclusion Unraveling the diversity of antibiotic resistance elements on the environment could not only identify new genes and mechanisms in which bacteria can resist to antibiotics, but also contribute to biotechnology processes, such as in bioremediation.
650 $aantibiotic resistance
650 $asoil
650 $aSolo
653 $aAromatic metabolism
653 $aAromatic metabolismo
653 $aDioxygenase
653 $aDioxygenase Metagenome
653 $aMetagenome
653 $aResistência aos antibióticos
700 1 $aISTVAN, P.
700 1 $aNORONHA, E. F.
700 1 $aQUIRINO, B. F.
700 1 $aKRÜGER, R. H.
773 $tBiotechnology Letters$gv. 37, n. 9, p.1809-1817, 2015.
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